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アッセイ
≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)
テクニック
cell based assay: suitable
検出方法
fluorometric
輸送温度
ambient
詳細
Hypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.
The BioTracker TP-HOCL1 dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. 2- mercaptoethanol and 1, 2-ethanedithiol were employed to protect the ketone of acedan in the design of HOCl probes. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement
Spectral Properties
Absorbance: 375nm
Emission: 500nm
References
Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8.
The BioTracker TP-HOCL1 dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. 2- mercaptoethanol and 1, 2-ethanedithiol were employed to protect the ketone of acedan in the design of HOCl probes. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement
Spectral Properties
Absorbance: 375nm
Emission: 500nm
References
Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8.
アプリケーション
Live cell fluorescent imaging
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
品質
Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 258.36 g/mol
Molar Mass: 258.36 g/mol
物理的形状
Lyophilized
保管および安定性
Store BioTracker TP-HOCL1 Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SCT043:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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