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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
9E10, monoclonal
形状
lyophilized
含まれません
preservative
化学種の反応性
rat (weakly), human, mouse (weakly)
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
アイソタイプ
IgG1
輸送温度
ambient
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MYC(4609)
mouse ... Myc(17869)
rat ... Myc(24577)
詳細
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
Recognizes a ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
This Anti-c-Myc (Ab-1) Mouse mAb (9E10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Chromatin IP, Paraffin Sections for the detection of c-Myc (Ab-1).
免疫原
Epitope: within amino acids 410-419
Human
a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
アプリケーション
Flow Cytometry (20 µg/ml or use Cat. No. OP10F)
Frozen Sections (2-10 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
Immunoprecipitation (1 µg/sample)
Chromatin Immunoprecipitation (5 µg/ml, see application references)
Paraffin Sections (not recommended)
Frozen Sections (2-10 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
Immunoprecipitation (1 µg/sample)
Chromatin Immunoprecipitation (5 µg/ml, see application references)
Paraffin Sections (not recommended)
警告
Toxicity: Standard Handling (A)
物理的形状
Lyophilized from a volatile buffer, 100 µg BSA.
再構成
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
アナリシスノート
Negative Control
HT1080 cells
HT1080 cells
Positive Control
HL-60 cells or lung carcinoma
HL-60 cells or lung carcinoma
その他情報
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.
The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
OP10L-MG:
OP10L-200UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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