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抗体製品の状態
purified antibody
品質水準
クローン
RPA34-20, monoclonal
形状
lyophilized
化学種の反応性
yeast, human, mouse
アイソタイプ
IgG2a
詳細
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the complexed (phosphorylated) and the ~34 kDa unphosphorylated subunit of replication protein A.
Recognizes the complexed (phosphorylated) and unphosphorylated subunits of replication protein A in HeLa and U293 cells and colon carcinoma tissue.
The replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.
免疫原
Epitope: within the p34 subunit of replication protein A
Human
replication protein A purified from U293 cells
アプリケーション
Immunoblotting (5 μg/ml)
Immunofluorescence (2.5 μg/ml)
Immunoprecipitation (1 μg/reaction)
Paraffin Sections (2.5 μg/ml, heat pre-treatment required)
Immunofluorescence (2.5 μg/ml)
Immunoprecipitation (1 μg/reaction)
Paraffin Sections (2.5 μg/ml, heat pre-treatment required)
物理的形状
Lyophilized from 20 mM ammonium bicarbonate solution, 100 μg BSA.
再構成
We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 μg/ml; product will be more stable
アナリシスノート
Positive Control
HeLa or U293 cells or colon carcinoma tissue
HeLa or U293 cells or colon carcinoma tissue
その他情報
Replication protein A (Ab-3) will react with both the complexed (phosphorylated) form of the protein as well as the free (unphosphorylated) 34 kDa subunit. Replication protein A can be used as a marker for cell division. Antibody should be titrated for op
保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
NA19L-MG:
NA19L-100UG:
NA19L-ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
S Din et al.
Genes & development, 4(6), 968-977 (1990-06-01)
Replication factor A (RF-A) is a multisubunit, cellular protein that functions with SV40 T antigen during the initiation stage of DNA replication at the SV40 origin. It also cooperates with other replication factors to stimulate the activity of both polymerases
S J Brill et al.
Nature, 342(6245), 92-95 (1989-11-02)
Cell-free replication systems for simian virus 40 (SV40) DNA are taken to be a model for the replication of eukaryotic chromosomes, because only one viral protein is required to supplement the replication proteins provided by a human cell extract. To
Initiation of eukaryotic DNA replication in vitro.
B Stillman
Annual review of cell biology, 5, 197-245 (1989-01-01)
T Tsurimoto et al.
The EMBO journal, 8(12), 3883-3889 (1989-12-01)
DNA synthesis by two eukaryotic DNA polymerases, alpha and delta, was studied using a single-strand M13 DNA template primed at a unique site. In the presence of low amounts of either DNA polymerase alpha or delta, DNA synthesis was limited
C R Wobbe et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(7), 1834-1838 (1987-04-01)
Simian virus 40 (SV40) DNA replication dependent on the SV40 origin of replication and the SV40 large tumor (T) antigen has been reconstituted in vitro with purified protein components isolated from HeLa cells. In addition to SV40 T antigen, these
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