MABT882

Anti-Prodynorphin (Dynorphin B29) Antibody, clone Dyn9

clone Dyn9, from mouse

• 別名: Beta-neoendorphin-dynorphin, Preprodynorphin, Preproenkephalin-B, Proenkephalin-B, Dynorphin B-29, Leumorphin
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: Dyn9, monoclonal
• 化学種の反応性: human, mouse, rat
• テクニック: immunohistochemistry: suitable (paraffin)
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_062247
• UniProtアクセッション番号: P06300
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Pdyn(29190)

詳細

詳細

Proenkephalin-B (UniProt P06300; also known as Beta-neoendorphin-dynorphin, Preprodynorphin) is encoded by the Pdyn gene (Gene ID 29190) in rat species. Proenkephalin B is an opioid polypeptide hormone involved with chemical signal transduction and cell communication. Prodynorphin is a basic building-block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds critical in learning and memory. Prodynorphin is produced with a signal peptide (a.a. 1-21) and a propeptide (a.a. 22-163) sequence. Following the removal of the signal and propeptide sequences, the protein is further processed by proeolytic cleavages to form Alpha-neoendorphin (a.a. 166-175), Beta-neoendorphin (a.a. 166-174), Big dynorphin (a.a. 202-233; Big Dyn), Dynorphin A(1-17) (a.a. 202-218; Dyn-A17; Dynorphin A); Dynorphin A(1-13) (a.a. 202-214), Dynorphin A(1-8) (a.a. 202-209), Leu-enkephalin (a.a. 202-206 & 221-225), Leumorphin (Dynorphin B-29) (a.a. 221-248), and Rimorphin (a.a. 221-233; Dynorphin B; Dyn-B; Dynorphin B(1-13)).

特異性

Clone Dyn9 recognizes preproenkephalin-B (a.a. 1-248 of rat sequence), proenkephalin-B (a.a. 22-248 of rat sequence), and leumorphin/dynorphin B-29 (a.a. 221-248 of rat sequence), but not other peptides derived from Proenkephalin-B.

免疫原

Thyroglobulin-conjugatged linear peptide corresponding to rat leumorphin (dynorphin B-29) C-terminal half sequence.

アプリケーション

Immunohistochemistry Analysis: A 1:20 dilution from a representative lot detected Prodynorphin/Dynorphin B29 immunoreactivity in human midbrain tissue sections.

Immunohistochemistry Analysis: A 1:20 dilution of clone Dyn9 hybridoma culture supernatant detected Prodynorphin/Dynorphin B29 immunoreactivity in human (hippocampus and cerebral cortex), mouse (fundus striati), and rat (nucleus supraopticus, lateral hipothalamic nucleus, paraventricular nucleus, and nucleus accumbens, septum pellucidum, caudate-putamen, and hippocampus) brain tissue sections (Courtesy of Ricardo Martínez Murillo, PhD, Cajal Institute, Madrid Spain).

This mouse monoclonal Anti-Prodynorphin (Dynorphin B29), clone Dyn9 Antibody, Cat. No. MABT882 is validated for use in Immunohistochemistry (Paraffin) for the detection of prodynorphin and leumorphin/dynorphin B-29.

品質

Evaluated by Immunohistochemistry in human thalamus tissue sections.

Immunohistochemistry Analysis: A 1:20 dilution of this antibody detected Prodynorphin/Dynorphin B29 immunoreactivity in human thalamus tissue sections.

ターゲットの説明

28.08/28.06/28.39 kDa (Rat/Mouse/Human preproenkephalin-B), 25.85/25.76/26.28 kDa (Rat/Mouse/Human proenkephalin-B), 3.320/3.320/3.352 kDa (Rat/Mouse/Human leumorphin) calculated.

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable