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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
BG8, monoclonal
分子量
calculated mol wt 62.82 kDa
observed mol wt ~62 kDa
精製方法
using protein G
交差性
mouse
交差性（ホモロジーによる予測）
human
包装
antibody small pack of 100 μL
テクニック
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
アイソタイプ
IgG1κ
エピトープ配列
N-terminal
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SHC1(6464)
詳細
SHC-transforming protein 1 (UniProt: P29353; also known as SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID: 6464) in human. p66Shc is a splice variant of p52Shc/p46Shc with a typical domain organization that is shared by all members of the Shc family of adaptor proteins. It has a phosphotyrosine binding domain, a collagen homology 1 region, and an SH2 domain. p66Shc is shown to be a downstream target of p53 and activated p53 induces its up-regulation by increasing its stability. p66Shc is suggested to be a genetic determinant of aging in mammals as its homozygous deletion is reported to delay senescence and prolong life span in mice. It regulates the mitochondrial pathway of apoptosis by inducing mitochondrial damage after dissociation from an inhibitory protein complex. Under conditions of excessive reactive oxygen species (ROS), p66Shc is phosphorylated on serine 36 by protein kinase C and is targeted to the mitochondrial intermembrane space where it serves as a redox enzyme by oxidizing cytochrome c and reducing oxygen to form ROS. In the absence of p66Shc, release of cytochrome c from mitochondria is impaired and apoptosome can only be activated if purified cytochrome c is supplied. p66Shc induces the collapse of the mitochondrial trans-membrane potential after oxidative stress. p66Shc is also shown to have a neuroprotective role in a murine model of multiple sclerosis. p66Shc knockout (p66-KO) mice develop typical signs of experimental autoimmune encephalomyelitis (EAE). (Ref.: Su, KG., et al. (2012). Eur. J. Neurosci. 35; 562-571; Orsini, F., et al. (2004). J. Biol. Chem. 279(24); 25689-25695).
特異性
Clone BG8 is a mouse monoclonal antibody that detects p66Shc. It was generated in p66Shc-/- mice. It targets an epitope within the N-terminal region.
免疫原
GST-tagged recombinant fragment corresponding to the first 110 amino acids from the N-terminal region of human SHC-transforming protein 1 (p66Shc).
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected SHC-transforming protein 1 in NIH3T3 cell lysate.
Tested Applications
Immunofluorescence Analysis: A representative lot detected p66shc 1 in Immunofluorescence application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).
Immunocytochemistry Analysis: A representative lot detected p66shc in Immunocytochemistry application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).
Western Blotting Analysis: A representative lot detected p66shc in Western Blotting application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95); Su, K.G., et al. (2012). Eur J Neurosci. 35(4):562-71).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected SHC-transforming protein 1 in NIH3T3 cell lysate.
Tested Applications
Immunofluorescence Analysis: A representative lot detected p66shc 1 in Immunofluorescence application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).
Immunocytochemistry Analysis: A representative lot detected p66shc in Immunocytochemistry application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).
Western Blotting Analysis: A representative lot detected p66shc in Western Blotting application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95); Su, K.G., et al. (2012). Eur J Neurosci. 35(4):562-71).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
物理的形状
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
再構成
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommended storage: +2°C to +8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABS2309-100UL:
MABS2309-25UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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