This item can only be ordered on the Merck Millipore website.
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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
LX015.1, monoclonal
交差性
mouse, human
テクニック
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MYBL2(4605)
詳細
B-Myb, also known as B-Myb or Myb-like protein 2, and encoded by the gene MYBL2/BMYB, is a transcription factor protein involved in the regulation of cell survival, proliferation and differentiation. B-Myb is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. B-Myb is a component of the DREAM complex (also named LINC complex) that is composed of at least E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2 proteins. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL22. B-Myb is localized to the nucleus. B-Myb is widely expressed and up regulated in cancer cells. B-Myb is an oncogenic transcription factor involved in G2/M progression and is a key target of anti-cancer therapy.
免疫原
Recombinant protein corresponding to human B-Myb.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
転写因子
転写因子
Detect B-Myb using this Anti-B-Myb Antibody, clone LX015.1 validated for use in western blotting.
Western Blotting Analysis: A representative lot from an independent laboratory detected U2OS cell lysates expressing B-Myb in U2OS (Chen, X., et al. (2013). Mol Cel Biol. 33(2):227-236.).
Western Blotting Analysis: A representative lot from an independent laboratory in NIH/3T3 cell lysates and in undifferentiated and differentiated F9 cell lysates (Knight, A. S., et al. (2009). Oncogene. 28(15):1737-1747.).
Western Blotting Analysis: A representative lot from an independent laboratory in cycling NIH/3T3 and F9 nuclear extracts (Muller, G. A., et al. (2012). Nucleic Acids Res. 40(4):1561-78.).
Western Blotting Analysis: A representative lot from an independent laboratory detected B-Myb wild-type cycling MEF cell lysates and not in B-Myb wild type G0 phase arrested MEF cell lysates. The same lot also detected B-Myb in G0 phase arrested and in cycling MEF cell lysates inappropriately expressing homozygous mutant B-Myb (Tavner, F., et al. (2007). Oncogene. 26(19): 2727-2735.).
Western Blotting Analysis: A representative lot from an independent laboratory in NIH/3T3 cell lysates and in undifferentiated and differentiated F9 cell lysates (Knight, A. S., et al. (2009). Oncogene. 28(15):1737-1747.).
Western Blotting Analysis: A representative lot from an independent laboratory in cycling NIH/3T3 and F9 nuclear extracts (Muller, G. A., et al. (2012). Nucleic Acids Res. 40(4):1561-78.).
Western Blotting Analysis: A representative lot from an independent laboratory detected B-Myb wild-type cycling MEF cell lysates and not in B-Myb wild type G0 phase arrested MEF cell lysates. The same lot also detected B-Myb in G0 phase arrested and in cycling MEF cell lysates inappropriately expressing homozygous mutant B-Myb (Tavner, F., et al. (2007). Oncogene. 26(19): 2727-2735.).
品質
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected B-Myb in 10 μg of HEK293 cell lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected B-Myb in 10 μg of HEK293 cell lysate.
ターゲットの説明
~80 kDa observed
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE886:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ruchi Kumari et al.
Scientific reports, 11(1), 21506-21506 (2021-11-04)
Cellular senescence is a stable cell cycle arrest that normal cells undergo after a finite number of divisions, in response to a variety of intrinsic and extrinsic stimuli. Although senescence is largely established and maintained by the p53/p21WAF1/CIP1 and pRB/p16INK4A
Pirunthan Perampalam et al.
The Journal of clinical investigation, 131(4) (2021-01-15)
DREAM (Dp, Rb-like, E2F, and MuvB) is a transcriptional repressor complex that regulates cell proliferation, and its loss causes neonatal lethality in mice. To investigate DREAM function in adult mice, we used an assembly-defective p107 protein and conditional deletion of
Benjamin B Morris et al.
Frontiers in oncology, 10, 585551-585551 (2021-01-26)
It has long been recognized that defects in cell cycle checkpoint and DNA repair pathways give rise to genomic instability, tumor heterogeneity, and metastasis. Despite this knowledge, the transcription factor-mediated gene expression programs that enable survival and proliferation in the
David Gallo et al.
Nature, 604(7907), 749-756 (2022-04-22)
Amplification of the CCNE1 locus on chromosome 19q12 is prevalent in multiple tumour types, particularly in high-grade serous ovarian cancer, uterine tumours and gastro-oesophageal cancers, where high cyclin E levels are associated with genome instability, whole-genome doubling and resistance to
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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