MABE1795

Anti-PP4C Antibody, clone 7B5

clone 7B5, from mouse

• 別名: Serine/threonine-protein phosphatase 4 catalytic subunit, EC: 3.1.3.16, Pp4, PPP4C, Protein phosphatase X, PP-X
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 7B5, monoclonal
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: bovine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• 包装: antibody small pack of 25 μL
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_002711.1
• UniProtアクセッション番号: P60510
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPP4C(5531)

詳細

詳細

Serine/threonine-protein phosphatase 4 catalytic subunit (UniProt: P60510; also known as EC: 3.1.3.16, PP4C, Pp4, Protein phosphatase X, PP-X) is encoded by the PPP4C (also known as PPP4, PPX) gene (Gene ID: 5531) in human. Protein phosphatase 4 (PP4), a ubiquitous serine/threonine phosphatase, is involved in the regulation of many cellular processes. It functions independently of protein phosphatase 2A (PP2A). Mammalian PP4 contains two regulatory subunits (R1 and R2) that interact with the catalytic subunit (PP4C)) and control its activity. Methylation at the C-terminal Leucine 307 is critical for interactions with regulatory subunits. PP4C-R2 complexes are reported to be involved in organelle assembly, maturation of the centrosome, apoptosis, DNA repair, and spliceosomal assembly. It is also reported to play a role in cell migration and in activation of c-Jun N-terminal kinase and NF-kappa B. PP4C has multiple metal ion binding sites and can bind two manganese ions per subunit. (Ref.: Cohen, PTW., et al. (2005). FEBS Lett. 579(15); 3278-3286).

特異性

Clone 7B5 specifically detects human Serine/threonine-protein phosphatase 4 catalytic subunit (PP4C). It targets an epitope within 17 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide coreesponding to 17 amino acids from the C-terminal region of human Serine/threonine-protein phosphatase 4 catalytic subunit (PP4C).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-PP4C, clone 7B5, Cat. No. MABE1795, is a highly specific mouse monoclonal antibody that targets Serine/threonine-protein phosphatase 4 catalytic subunit and has been tested for use in Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected PP4C in lysate from NIH3T3 mouse fibroblasts ectopically expressing N-terminally tagged human PP4 catalytic subunit (PPP4C).(Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).

品質

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

ターゲットの説明

35.08 kDa calculated.

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.