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由来生物
rat
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
5D6, monoclonal
交差性
mouse
テクニック
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG2aκ
NCBIアクセッション番号
UniProtアクセッション番号
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Tet1(52463)
詳細
Methylcytosine dioxygenase TET1 (EC 1.14.11.n2; UniProt Q3URK3; also known as CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog) is encoded by the Tet1 (also know as Cxxc6, Kiaa1676) gene (Gene ID 52463) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.
特異性
Clone 5D6 detected exogenously expressed TET1, but not TET2 or TET3, GST fusion protein (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
免疫原
Epitope: Region between DNA-binding and 2-oxoglutarate-binding domains
His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET1.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
エピジェネティクス
Anti-TET1 Antibody, clone 5D6 is an antibody against TET1 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.
Western Blotting Analysis: A representative lot detected the endogenous TET1 in murine embryonic stem cell (mESC) lysate, as well as exogenously expressed TET1, but not TET2 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET1 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Anaylsis: Clone 5D6 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET1 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET1 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Anaylsis: Clone 5D6 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET1 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
品質
Evaluated by Immunocytochemistry in mouse ESCs.
Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TET1 in mouse embryonic stem cells (ESCs).
Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TET1 in mouse embryonic stem cells (ESCs).
ターゲットの説明
219.3 calculated.
関連事項
Replaces: ABE1034
物理的形状
Protein G purified.
Format: Purified
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE1144:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Linlin Liu et al.
Frontiers in cell and developmental biology, 9, 644135-644135 (2021-04-10)
Tet enzymes participate in DNA demethylation and play critical roles in stem cell pluripotency and differentiation. DNA methylation alters with age. We find that Tet1 deficiency reduces fertility and leads to accelerated reproductive failure with age. Noticeably, Tet1-deficient mice at
Benedict Shi Xiang Lian et al.
iScience, 25(4), 104118-104118 (2022-04-12)
The innate immune system is an immediate defense against infectious pathogens by the production of inflammatory cytokines and other mediators. Deficiencies of epigenetic regulatory enzymes, such as Tet1 and Dnmt1, cause dysregulation of cytokine expression. However, it is unclear if
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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