MABC592

Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free

clone TA99, 1 mg/mL, from mouse

• 別名: 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA oxidase, Catalase B, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1, TRP, TRP-1, TRP1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: TA99, monoclonal
• 化学種の反応性: human, mouse
• 濃度: 1 mg/mL
• テクニック: blocking: suitable (interfering antibodies); flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_000541
• UniProtアクセッション番号: P17643
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TYRP1(7306)

詳細

詳細

TRP1/TYRP1, also known as 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA, Catalase B, Glycoprotein 75, Melanoma antigen gp75, or Tyrosinase-related protein 1 (TRP/TRP-1/TRP1), and encoded by the gene TYRP1/CAS2/TYRP/TYRRP, is involved in melanin synthesis and is the enzyme that oxidizes DHICA into indole-5,6-quinone-2-carboxylic acid in the pathway. TRP1/TYRP1 is critical for skin/eye/hair pigmentation. Mutations are associated with Albinism. TRP1/TYRP1 is regulated by the microphthalmia-associated transcription factor (MITF). MITF is a master regulator of pigmentation and a target of β-catenin in Wnt/β-catenin signaling during melanocyte differentiation. TRP1/TYRP1 is also regulated by the microRNA miR-145, and this microRNA is being examined as a possible therapy for hyperpigmentation. TRP1/TYRP1 is expressed as a membrane protein in melanosomes and endosomes in pigment cells.

免疫原

Whole cells corresponding to human TRP1/TYRP1.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
アポトーシス-追加

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TRP1/TYRP1 in human melanoma tissue.

Blocking of Interferring Antibodies Analysis: A representative lot from an independent laboratory suppressed the growth of subcutaneous B16 tumors (Patel, D., et al. (2008). Anticancer Res. 28(5A):2679-2686.).

Activity Assay Analysis: A representative lot from an independent laboratory improves anti-tumor efficacy by augmenting systemic CD8+T cell responses to tumor cells (Saenger, Y. M., et al. (2008). Cancer Res. 68(23):9884-9891.).

Immunoprecipiptation Analysis: A representative lot from an independent laboratory immunoprecipiated TRP1/TYRP1 from B16 cell lysate (Srinivasan, R., et al. (2002). Cancer Immun. 19(2):8.).

This Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free is validated for use in western blotting, IHC, blocking of inteferring antibodies, IP & flow cytometry for the detection of TRP1/TYRP1.

品質

Evaluated by Western Blottng in mouse skin tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TRP1/TYRP1 in 10 µg of mouse skin tissue lysate.

ターゲットの説明

~60 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable