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由来生物
rat
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
6D8, monoclonal
分子量
calculated mol wt 40.53 kDa
observed mol wt ~40 kDa
精製方法
using protein G
化学種の反応性
human
包装
antibody small pack of 100 μL
テクニック
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
アイソタイプ
IgG2a
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
輸送温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
rat ... AIFM2(84883)
関連するカテゴリー
詳細
Ferroptosis suppressor protein 1 (UniProt: Q9BRQ8; also known as FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, AMID, p53-responsive gene 3 protein, AIFM2) is encoded by the AIFM2 (also known as AMID, PRG3) gene (Gene ID: 84883) in human. AIFM2 is a lipid-anchor membrane protein of the FAD-dependent oxidoreductase family that is also detected in the nucleus and mitochondrial membrane. It is highly expressed in heart and moderately in liver and skeletal muscle. Low expression levels are observed in placenta, lung, kidney, and pancreas. It is reported to be down-regulated in a number of human tumors. It serves as a NAD(P)H-dependent oxidoreductase and is involved in cellular oxidative stress response. At the plasma membrane, it catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. It is shown to cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis and its anti-ferroptotic function is reported to be independent of cellular glutathione levels. It also plays a role in mitochondrial stress signaling. Upon induction of oxidative stress, it associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct. This HNE adduct inactivates the NADH oxidoreductase activity of AIFM2 and facilitates its translocation from mitochondria into the nucleus triggering DNA damage and cell death. (Ref.: Doll, S., et al. (2019). Nature 575(7784); 693-698; Miriyala, S., et al. (2016). Free Radic. Biol. Med. 91; 68-80).
特異性
Clone 6D8 is a rat monoclonal antibody that detects human Ferroptosis suppressor protein 1 (AIFM2).
免疫原
SUMO-His-tagged full-length recombinant human Ferroptosis suppressor protein 1 (FSP1/AIFM2).
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected AIFM2 in A549 cell lysate.
Tested Appliacations
Western Blotting Analysis: A 1:500 dilution from a representative lot detected AIFM2 in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:25 Dilution from a representative lot detected AIFM2 in HepG2 cells.
Immunofluorescence Analysis: A representative lot detected AIFM2 in Immunofluorescence applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Western Blotting Analysis: A representative lot detected AIFM2 in Western Blotting applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
ELISA Analysis: A representative lot detected AIFM2 in ELISA applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected AIFM2 in A549 cell lysate.
Tested Appliacations
Western Blotting Analysis: A 1:500 dilution from a representative lot detected AIFM2 in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:25 Dilution from a representative lot detected AIFM2 in HepG2 cells.
Immunofluorescence Analysis: A representative lot detected AIFM2 in Immunofluorescence applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Western Blotting Analysis: A representative lot detected AIFM2 in Western Blotting applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
ELISA Analysis: A representative lot detected AIFM2 in ELISA applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-AIFM2, clone 6D8-11, Cat. No. MABC1638, is a rat monoclonal antibody that detects Ferroptosis suppressor protein 1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.
物理的形状
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABC1638-25UL:
MABC1638-100UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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