MAB80138 anti hMPV antibody was used in Immunofluorescence in the following study. A549 or WI-38 cells were fixed in PBS containing 4% paraformaldehyde for 10 minutes on ice, followed by permeabilization with 0.1% saponin for 30 minutes and blocking with PBS containing 10% fetal bovine serum and 2.5% bovine serum albumin for 60 minutes at room temperature. The primary antibody against hMPV nucleoprotein (N protein) was incubated overnight at 4°C, followed by secondary antibody GAM 647 for 30 minutes at room temperature. Nuclear staining was achieved with DAPI staining for 10 minutes. The study can be accessed at https://pmc.ncbi.nlm.nih.gov/articles/PMC5772477/.
This product is usually provided at a concentration of 1 mg/mL, and the lot-specific Certificates of Analysis can be found on the website: https://www.sigmaaldrich.com/product/Anti-Human-Metapneumovirus-Antibody-clone-507,MM_NF-MAB80138#anchor_COA
価格・在庫情報は確認できません。詳細については、Merckをご覧ください。
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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
507, monoclonal
交差性
human
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable
アイソタイプ
IgG2a
輸送温度
wet ice
詳細
Human metapneumovirus (hMPV) is a member of the pneumovirinae subfamily of paramyxoviruses, first described in 2001 from pediatric respiratory specimens in the Netherlands. It is an enveloped pleomorphic virus with a single negative-strand RNA genome. Two major serotypes, A and B, have been described. Several studies identified hMPV in specimens worldwide, and estimated that by age 5 years 70% of children have developed antibodies to hMPV.
特異性
Recognizes the nuclear protein. Reactive with all hMPV sub-types; A1, A2, B1, B2.
アプリケーション
Detect Human Metapneumovirus using this Anti-Human Metapneumovirus Antibody, clone 507 validated for use in ELISA, FC, IF & WB.
物理的形状
Format: Purified
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB80138:
MAB80138-K:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Estimates of individuals exposed to human metapneumovirus in a community-based Taiwanese population in 1999.
Chung-Guei Huang,Kuo-Chien Tsao,Tzou-Yien Lin,Yhu-Chering Huang,Li-Ang Lee,Tzu-Hou Chen et al.
Archives of Virology null
Simon Loevenich et al.
Mediators of inflammation, 2019, 4964239-4964239 (2019-11-07)
Human metapneumovirus (HMPV) may cause severe respiratory disease. The early innate immune response to viruses like HMPV is characterized by induction of antiviral interferons (IFNs) and proinflammatory immune mediators that are essential in shaping adaptive immune responses. Although innate immune
Xiaoyan Li et al.
Intervirology, 58(6), 403-412 (2016-04-21)
No licensed vaccines or therapeutic agents for human metapneumovirus (hMPV) infection exist to date. We aimed to construct a multi-epitope peptide (MEP) of hMPV to show promising results for epitope-based vaccine development. Six independent algorithms were screened to predict B-cell
Youxian Li et al.
Scientific reports, 8(1), 883-883 (2018-01-19)
Thymic stromal lymphopoietin (TSLP) is associated with several allergic diseases including asthma. Two isoforms of TSLP exist in humans, a long form (lfTSLP) and a short form (sfTSLP), displaying distinct immunological functions. Recently, TSLP was found to be upregulated in
Mijia Lu et al.
Nature microbiology, 5(4), 584-598 (2020-02-06)
Internal N6-methyladenosine (m6A) modification is one of the most common and abundant modifications of RNA. However, the biological roles of viral RNA m6A remain elusive. Here, using human metapneumovirus (HMPV) as a model, we demonstrate that m6A serves as a
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Regarding MAB80138, anti hMPV antibody, is there any paper that was published using this antibody for Immunofluorescence? In addition was is the approximate concentration of the antibody that you will send?
1 回答-
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