MAB4385

Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85

clone TRA-1-85, Chemicon^®, from mouse

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: TRA-1-85, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 入力: sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• アイソタイプ: IgG1
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.

特異性

This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.

SPECIES REACTIVITIES:

Refer to comments regarding specificity

免疫原

2102Ep human Embryonal Carcinoma Cells

Epitope: blood group antigen Ok(a)

アプリケーション

Research Category
幹細胞研究

Research Sub Category
多能性及び初期分化

Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.

FACS Analysis: a starting range of 5-20 μg/ml is suggested.

Immunoprecipitation

Immunoblotting

Optimal working dilutions must be determined by the end user.

ターゲットの説明

35-69 kDa

物理的形状

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide

保管および安定性

Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months

アナリシスノート

Control
Hemopoietic cells and a wide range of normal human tissues

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable