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製品名
Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free, clone 15F11, Chemicon®, from mouse
由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
15F11, monoclonal
交差性
human
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ITGAV(3685)
特異性
Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. The antibody binds to the alphaVbeta5 complex. The monoclonal antibody MAB2019Z has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding of the antibody to alphaVbeta5. Monoclonal antibody MAB2019Z does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
免疫原
Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
インテグリン
インテグリン
Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC.
Immunoprecipitation
Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.
ELISA
Optimal working dilutions must be determined by end user.
Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.
ELISA
Optimal working dilutions must be determined by end user.
物理的形状
Format: Purified
Purified immunoglobulin. Liquid at 1 mg/mL in PBS, containing no preservatives.
保管および安定性
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB2019Z:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Khine Kyaw Oo et al.
BMC cancer, 21(1), 65-65 (2021-01-16)
Chemoresistance is one of the main problems in treatment of cancer. Periostin (PN) is a stromal protein which is mostly secreted from cancer associated fibroblasts in the tumor microenvironment and can promote cancer progression including cell survival, metastasis, and chemoresistance.
Stimulation of glioma cell motility by expression, proteolysis, and release of the L1 neural cell recognition molecule.
Yang, M; Adla, S; Temburni, MK; Patel, VP; Lagow, EL; Brady, OA; Tian, J; Boulos, MI; Galileo, DS
Cancer Cell International null
Daniel Vial et al.
Journal of cell science, 121(Pt 10), 1661-1670 (2008-05-01)
The plasminogen activation system regulates matrix remodeling through both proteolytic and non-proteolytic mechanisms. Studies were undertaken to determine the effects of the plasminogen activator inhibitor 1 (PAI1) on the assembly of the fibronectin matrix. The addition of PAI1 to MG-63
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The Journal of cell biology, 158(7), 1287-1297 (2002-10-03)
p21-activated kinase 1 (PAK1) can affect cell migration (Price et al., 1998; del Pozo et al., 2000) and modulate myosin light chain kinase and LIM kinase, which are components of the cellular motility machinery (Edwards, D.C., L.C. Sanders, G.M. Bokoch
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We previously reported 18FPRGD2 uptake by the coxofemoral lining, intervertebral discs and facet joint osteophytes in OA using PET/SCAN imaging. However, the molecular mechanism by which the PRGD2 tracer interacts with joint tissues and osteophytes in OA remains unclear. As
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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