This item can only be ordered on the Merck Millipore website.
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由来生物
mouse
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
5D4E11B6, monoclonal
交差性
human, mouse, rat
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ADAMTS1(9510)
詳細
ADAMTS-1 is a member of a family of extracellular proteases known as ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs). Members of the ADAMTS family share distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TSP) motif. Individual members of this family differ in the number of C-terminal TSP motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TSP motifs. ADAMTS-1 cleaves aggrecan, a cartilage proteoglycan and appears to demonstrate angiogenesis inhibitory activity. The maturation of ADAMTS-1 requires two independent and sequential processing events that may release two forms of the protein. With the cleavage of the prodomain from the 110 kDa zymogen, an 87 kDa active form remains which can be further processed by removal of the catalytic subunit from the TSP repeats, leaving the C-termianl 65 kDa soluble form. The proteolytic deletion of the last two TSP repeats, is probably significant for the in vivo function of this protein.
特異性
Clone 5D4E11B6 recognizes human ADAMTS-1.
Reactivity with other species has not been tested.
免疫原
Myc-His carboxyl tagged full-length recombinant protein based on the human sequence.
アプリケーション
Detect ADAMTS-1 using this Anti-ADAMTS-1 Antibody, clone 5D4E11B6 validated for use in ELISA, IP & WB.
Western blot: 1:2000 - 1:4000 dilution on A431 cell lysate
Note: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms.
Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate
Note: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms.
Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate
ターゲットの説明
110 / 87 / 65 kDa
物理的形状
Ascites. Liquid containing 0.05% NaN3.
アナリシスノート
Control
A431 cell lysate
HEK293 cell lysate
PC-12 cell lysate
Embryonic mouse brain lysate
MCF-7 cell lysate
A431 cell lysate
HEK293 cell lysate
PC-12 cell lysate
Embryonic mouse brain lysate
MCF-7 cell lysate
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1810:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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