CBA024

InnoZyme Myeloperoxidase Activity Kit

• 別名: InnoZyme MPO Activity Kit
• UNSPSCコード: 41116133
• NACRES: NA.77

特性

• 使用法: sufficient for 96 tests
• 品質水準: 100
• 化学種の反応性: human
• 包装: pkg of 1 96-well plate(s)
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• assay range: sensitivity: 330 pg/mL; standard curve range: 5-100 ng/mL
• 入力: sample type serum; sample type cerebrospinal fluid (CSF); sample type cell lysate
• 検出方法: colorimetric
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

The InnoZyme Myeloperoxidase Activity Kit is a specific and sensitive assay designed to measure myeloperoxidase activity and to screen enzyme inhibitors. The assay uses a polyclonal antibody specific for human MPO immobilized on a 96-well plate to specifically capture the enzyme. Activity of captured myeloperoxidase is measured using a detection reagent that includes TMB and hydrogen peroxide. Following color development, the reaction is stopped with sulfuric acid and the absorbance of the oxidized TMB is detected at 450 nm.

構成

Anti-MPO Coated 96-Well Plate, MPO Standard, TMB, Hydrogen Peroxide, Assay Buffer, Sample Buffer, Stop Solution, Plate Sealers, and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

規格

Assay Time: 2 h

原理

The Calbiochem InnoZyme Myeloperoxidase Activity Kit is designed to measure human myeloperoxidase activity in cell lysates and biological samples and to screen enzyme inhibitors.

調製ノート

All reagents necessary to perform the assay are supplied with the kit. Equilibrate all reagents to room temperature (15-25°C) just prior to use.Sample Buffer (1X): Dilute Sample Buffer 1:20 by adding 25 ml Sample Buffer to 475 ml dH₂O. Myeloperoxidase Standard (1g/ml): Dilute the Myeloperoxidase Standard 1:100 by adding 5 l Myeloperoxidase Standard to 495 l chilled Sample Buffer (1X) obtain a concentration of 1 g/ml. Maintain the diluted Myeloperoxidase Standard (1 g/ml) on ice. Note: Following initial thaw the Myeloperoxidase Standard should be dispensed into aliquots and stored at -20°C. Avoid freeze/thaw cycles.<div class="Bio_doc_image">
Table 1: Myeloperoxidase Standard Dilutions
Prepare Myeloperoxidase Standard dilutions in the range of 5-100 ng/ml using the above guidelines.
</div>Hydrogen Peroxide (0.3%): Dilute the Hydrogen Peroxide (30%) 1:100 by adding 5 l Hydrogen Peroxide (30%) to 495 l chilled dH₂O. Note: A total of 40 l is required to cover the entire Coated 96-Well Plate. If the entire plate is not to be used at one time, prepare fresh Hydrogen Peroxide (0.3%) each time the assay is performed. Following initial thaw the Hydrogen Peroxide should be dispensed into aliquots and stored at -20°C. Avoid freeze/thaw cycles.TMB Detection Reagent: Note: The desired amount of TMB Detection Reagent should be prepared fresh, just prior to use. To prepare TMB Detection Reagent, mix the following components:

• 7.16 ml dH₂O
• 2.00 ml Assay Buffer
• 0.80 ml TMB
• 40 l H₂O₂(0.3%)

Vortex gently 3 X 3 sTo prepare TMB Detection Reagent for a variable number of strips, use the following guidelines: <div class="Bio_doc_image">Table 2: Preparation of TMB Detection Reagent
To prepare TMB Detection Reagent for a variable number of strips, use the above guidelines.
</div>

Dilute samples with Sample Buffer (1X) as needed. Note: If sample dilution is not required for sensitivity reasons, prepare the sample by adding 475 µl sample to 25 µl 20X Sample Buffer to ensure appropriate pH for the first incubation.

保管および安定性

Upon arrival, store the unopened kit at -20°C. All kit components, once opened, can be stored for up to 3 months under the following conditions:



Store at 4°C: TMB, Anti-MPO Coated 96-Well Plate, Assay Buffer, Stop Solution, and Sample Buffer

Store at -20°C: Myeloperoxidase Standard, and Hydrogen Peroxide



Note: Following initial use the Myeloperoxidase Standard and the Hydrogen Peroxide should be dispensed into aliquots and stored at -20°C. Avoid freeze/thaw cycles.

アナリシスノート

Positive Control
Human myeloperoxidase

その他情報

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Weil, S.C., et al. 1988. Science 240, 790.
Winterbourn, C.C., et al. 2000. Curr. Opin. Hematol. 7, 53.
Brennan, M.L., et al. 2003. New Engl. J Med. 349, 1595.
Zheng, L., et al. 2004. J. Biol. Chem. in press
Vita, J.A., et al. 2004. Circulation, 110, 1134.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H290,H315,H317,H318
• 注意書き: P234 - P261 - P264 - P280 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1
• 保管分類コード: 8A - Combustible corrosive hazardous materials