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化学種の反応性（ホモロジーによる予測）
mammals
メーカー／製品名
Chemicon®
テクニック
activity assay: suitable
UniProtアクセッション番号
検出方法
colorimetric
輸送温度
dry ice
詳細
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.
アプリケーション
Research Category
アポトーシス及び癌
アポトーシス及び癌
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
構成
5X Cell Lysis Buffer (Part No. 90065): 5 mL
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90082): 1 mL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90082): 1 mL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
保管および安定性
Store kit materials at -20°C up to their expiration date.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
消防法
第4類:引火性液体
第三石油類
危険等級III
非水溶性液体
Jan Code
APT171:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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