C3556

カタラーゼ ヒト赤血球由来

≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein

• 別名: H₂O₂:H₂O₂ オキシドレダクターゼ
• CAS番号: 9001-05-2
• NACRES: NA.54
• UNSPSC Code: 12352204
• EC Number: 232-577-1
• MDL number: MFCD00081483
• EC Number: 1.11.1.6 (BRENDA, IUBMB)
• eCl@ss: 32160410
• Specific activity: ≥30,000 units/mg protein
• Assay: ≥90% (SDS-PAGE)
• Biological source: human erythrocytes
• Concentration: ≤10 mg/mL

特性

• biological source: human erythrocytes
• Quality Segment: 200
• assay: ≥90% (SDS-PAGE)
• form: buffered aqueous solution
• specific activity: ≥30,000 units/mg protein
• mol wt: tetramer ~250 kDa
• concentration: ≤10 mg/mL
• technique(s): cell based assay: suitable
• UniProt accession no.: P04040
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: O(CC)C(=O)c1ccc(cc1)O
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChI key: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• Gene Information: human ... CAT(847)

詳細

General description

Research area: Cell Signaling

Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule.[1] It is a ubiquitous enzyme found in most aerobic organisms.[2] The catalase (CAT) gene is located on the human chromosome at 11p13.[3]

Application

Catalase from human erythrocytes has been used:

• to prevent reoxidation of reduced cytochrome c by H2O2 while measuring the production of superoxide with cytochrome C.[4]
• as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of human skin fibroblasts[5]
• as a component of the gloxy mix for single-molecule imaging[6]

Biochem/physiol Actions

カタラ-ゼは、活性酸素種である過酸化水素が水と酸素に分解する反応を活性化します。天然の抗酸化物質としても機能し、タンパク質、脂質および核酸の酸化的損傷から細胞を保護します。また、活性酸素種が遺伝子発現とアポト-シスに果たす役割に関する研究にも用いられています。

Deficiency of catalase and oxidative stress are associated with vitiligo, hypertension, Alzheimer′s disease, diabetes mellitus, and acatalasemia. Catalase shows a dichotomous role in cancer as its expression is at a higher level in chronic lymphocytic leukemia, glioma, melanoma, and gastric cancer and lower levels of expression are seen in acute myeloid leukemia, pancreatic, lung, prostate, and skin (non-melanoma) cancers.[2]

Physical form

50 mM Trisバッファ-溶液、pH 8.0

Preparation Note

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Analysis Note

タンパク量はビウレット法で測定

Other Notes

1ユニットは、pH 7.0、25°Cで、H₂O₂濃度が10.3 mMから9.2 mMに低下する間に1分あたり1.0 μmolのH₂O₂を分解する酵素量であり、A₂₄₀の減少率で測定します。

安全性情報

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• 保管分類: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)