Purified mouse monoclonal antibody. Recognizes the ~120 kDa TLR7 protein.
Recognizes the ~120 kDa TLR7 protein in IMR32 cells.
This Anti-TLR7 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting for the detection of TLR7.
免疫原
A recombinant polypeptide corresponding to amino acids of 27-127 human TLR7, expressed as a GST fusion protein
アプリケーション
ELISA (see comments) Immunoblotting (1-5 g/ml)
警告
Toxicity: Regulatory Review (Z)
物理的形状
In PBS, pH 7.2.
再構成
Following initial thaw, aliquot and freeze (-20°C).
アナリシスノート
Positive Control IMR32 cells
その他情報
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Medical science monitor : international medical journal of experimental and clinical research, 26, e923104-e923104 (2020-05-27)
BACKGROUND The metabolic processing of ellagic acid (EA) by cytochrome P450s (CYP450s) expressed in the intestines is unclear. This study aimed to investigate the effects of CYP450s that are highly expressed in HIEC cells on metabolic activity of EA. MATERIAL