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由来生物
bovine
品質水準
アッセイ
>95% (total protein)
形状
liquid
メーカー／製品名
Chemicon®
テクニック
cell based assay: suitable
UniProtアクセッション番号
輸送温度
dry ice
遺伝子情報
bovine (calf) ... PRNP(281427)
詳細
Histidine-tagged full-length mature part of bovine PrP (25-244) is expressed in E. Coli BL21, solubilized from inclusion bodies in 6 M guanidine-HCl, and purified by Ni(II)-nitriloacetate agarose chromatography followed by reversed-phase HPLC (C4 column)
Prion diseases or transmissible spongiform encephalopathies are neurodegenerative diseases that affect both humans and animals (Prusiner 1998). All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPSc) (Pan et al. 1993). Both PrPSc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids (Basler et al. 1986). Many mammalian PrPs have a 22 amino acid N-terminal signal sequence (Hope et al. 1986; Turk et al. 1988) and 23 amino acid C-terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor (Stahl et al. 1987, 1990). The mature protein of 209 amino acids contains one disulfide bond (Turk et al. 1988) and has two sites of asparagine-linked glycosylation (Endo et al. 1989; Oesch et al. 1995).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経変性疾患
神経変性疾患
Antigen in standard immunochemical detection of BSE.
Optimal working dilution must be determined by the end user.
Optimal working dilution must be determined by the end user.
物理的形状
Liquid in 10 mM sodium acetate buffer, pH 4.0, containing 0.01% sodium azide.
保管および安定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
アナリシスノート
The PrPc appears as a single band of about 27 kD by SDS-PAGE
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AG210:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Conversion of alpha-helices into beta-sheets features in the formation of the scrapie prion proteins.
Pan, K M, et al.
Proceedings of the National Academy of Sciences of the USA, 90, 10962-10966 (1993)
Diversity of oligosaccharide structures linked to asparagines of the scrapie prion protein.
Endo, T, et al.
Biochemistry, 28, 8380-8388 (1989)
Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene.
Basler, K, et al.
Cell, 46, 417-428 (1986)
Purification and properties of the cellular and scrapie hamster prion proteins.
Turk, E, et al.
European Journal of Biochemistry, 176, 21-30 (1988)
Identification of glycoinositol phospholipid linked and truncated forms of the scrapie prion protein.
Stahl, N, et al.
Biochemistry, 29, 8879-8884 (1990)
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