Oligodendrocyte transcription factor 2 (UniProt Q9EQW6; also known as bHLHe19, Olg-2, Oligo2, RACK17, RK17) is encoded by the Olig2 (also known as Bhlhb1) gene (Gene ID 50913) in murine species. Olig2 is a basic helix-loop-helix (bHLH) transcription factor expressed in a spinal cord ventricular zone domain where it mediates the sequential generation of motoneurons and oligodendrocytes during embryogenesis. Olig2 also functions as an anti-neurogenic factor in pMN progenitors at early time points to sustain the cycling progenitor pool. The anti-neurogenicity of Olig2 is also responsible for its role in tumorigenesis, especially in brain tumors and leukemia. Due to its expression in glioblastoma and other diffuse gliomas, Olig2 is a useful diagnostic marker of these brain tumors.
免疫原
KLH-conjugated linear peptide corresponding to a sequence in the N-terminal region of mouse Olig-2.
アプリケーション
Anti-Olig-2 Antibody is an antibody against Olig-2 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin).
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Olig-2 in 500 µg of mouse brain tissue lysate. Immunohistochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in rat hindbrain tissue sections. Immunocytochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in PC12 cells.
品質
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Olig-2 in 10 µg of mouse brain tissue lysate.
ターゲットの説明
~37 kDa observed. 32.40 kDa calculated.
関連事項
Replaces: AB15328
物理的形状
Purified rabbit polyclonal antibody in PBS and 0.1 % sodium azide.
その他情報
Concentration: Please refer to lot specific datasheet.
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