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由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
human, mouse
化学種の反応性（ホモロジーによる予測）
horse (based on 100% sequence homology), monkey (based on 100% sequence homology)
テクニック
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... HOMER1(9456)
詳細
Homer protein homolog 1 (UniProt Q86YM7; also known as Homer-1, Homer1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID 9456) in human. Homer-1, -2, and -3 constitute a family of postsynaptic scaffold proteins that play a central role in calcium signaling. Homer members share a conserved N-terminal Ena/VASP homology 1 (EVH1) domain (a.a. 1-110 of Homer-1) that mediate signaling complexes assembly by interacting with PPXXFR proline-rich consensus sequences found in various other scaffolding and signal transduction molecules, including metabotropic glutamate receptors (mGluRs) and 1,4,5-trisphosphate receptors (IP3Rs). In addition to mediate group I mGluRs signaling via their EVH1 domain, Homer-1 spliced variants Vesl-1L, 1b, and 1d, but not Vesl-1M or Vesl-1S, also contain a C-terminal coiled-coil (a.a. 181-352) region that mediate self-multimerization. Homer-1 variants are associated with the etiology of many neurological diseases, such as chronic pain, mental retardation syndromes, Alzheimer′s disease (AD), Parkinson′s disease (PD), schizophrenia, drug-induced addiction, and traumatic brain injury (TBI). Homer1b/c-knockdown is reported to protect against chemically induced seizure in mice as a result of preventing mGluR5-mediated mTOR pathway activation.
特異性
Broad species reactivity expected based on high sequence homology.
This rabbit polyclonal antibody targets an internal sequence within the N-terminal half of human Homer1 isoform 1b and mouse isoform Vesl-1L. Immunogen sequence appears near the C-terminus of human isoform 1h and mouse isoforms 1b, 1d, Vesl-1M, and Vesl-1S, but absent in human isoform 1e. Highly homologous sequence (~83%) is also found in the N-terminal half of rat isoform 1c (Vesl-1L) and near the C-terminus of rat isoforms 1a (Vesl) and 1b.
免疫原
KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human Homer1 isoform 1b and near the C-terminus of isoform 1h.
アプリケーション
Anti-Homer1, Cat. No. ABN37, is a highly specific rabbit polyclonal antibody that targets Homer protein homolog 1 and has been tested in Immunohistochemistry and Western Blotting.
Immunohsitochemistry Analysis: A 1:500 dilution from a representative lot detected Homer1 in human and mouse cerebral cortex tissue sections.
Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).
Immunohsitochemistry Analysis: A representative lot detected significantly increased stratum radiatum and stratum oriens Homer1 immunoreactivity in floating brain sections from mice received AP-12 intraperitoneal injection (Jansone, B., et al. (2015). PLoS One. 10(6):e0127686).
品質
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Homer1 in 10 µg of mouse brain tissue lysate.
ターゲットの説明
~47 kDa observed. 40.28/19.86 kDa (human isoform 1b/1h), 40.22/42.08/41.41/22.78/20.91 kDa (mouse isoform 1b/1d/Vesl-1L/Vesl-1M/Vesl-1S) calculated. Uncharacterized bands may be observed in some lysate(s).
その他情報
Concentration: Please refer to lot specific datasheet.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABN37:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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