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由来生物
rabbit
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
交差性
mouse, human
テクニック
immunoprecipitation (IP): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SHARPIN(81858)
詳細
Sharpin, also known as Sharpin Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1, and encoded by the gene name SHARPIN/ SIPL1/ PSEC0216, is component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Sharpin is highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Sharpin is up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors
免疫原
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of human Sharpin.
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
Research Sub Category
免疫グロブリン及び免疫
免疫グロブリン及び免疫
This Anti-Sharpin antibody is validated for use in WB, IP for the detection of Sharpin.
Western Blotting Analysis: A representative lot detected Sharpin in Splenic B cells were treated with an anti-CD40 antibody, anti-NEMO immunoprecipitates (IP) from TNF-a-treated MEFs HEK293T, Jurkat, cpdm MEFs, Cpdm MEFs transfected with control or HOIL-1L-specific siRNA, Brain lysates of Wt and cpdm mice, transfected cpdm MEFs, Lysates from Wt mice organsand splenic B and T cells from Wt and cpdm mice (Tokunaga, F., et al. (2010). Nature. 471:633-636).
Western Blotting Analysis: A representative lot detected Sharpin in HeLa cell lysate (Li, S., et al. (2013). Nature. 242-246).
Western Blotting Analysis: A representative lot detected Sharpin in HOIP-null MEFs, HOIP WT MEFs or HOIP N84A/Y93A MEFs (Takiuchi, T., et al. (2014). Genes to Cells. 1-19).
Western Blotting Analysis: A representative lot detected Sharpin in MEFs, BMDMs, and primary keratinocytes
from WT mice (Tamiya, H., et al. (2014). J. Immunology. 192:000-000).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sharpin in HEK293T (Tokunaga, F., et al. (2010). Nature. 471:633-636).
Western Blotting Analysis: A representative lot detected Sharpin in HeLa cell lysate (Li, S., et al. (2013). Nature. 242-246).
Western Blotting Analysis: A representative lot detected Sharpin in HOIP-null MEFs, HOIP WT MEFs or HOIP N84A/Y93A MEFs (Takiuchi, T., et al. (2014). Genes to Cells. 1-19).
Western Blotting Analysis: A representative lot detected Sharpin in MEFs, BMDMs, and primary keratinocytes
from WT mice (Tamiya, H., et al. (2014). J. Immunology. 192:000-000).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sharpin in HEK293T (Tokunaga, F., et al. (2010). Nature. 471:633-636).
品質
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Sharpin in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Sharpin in 10 µg of HepG2 cell lysate.
ターゲットの説明
~40 kDa observed. Anti-SHARPIN antibody demonstrated an approximately 40 kDa protein, the band is consistent with the predicted 39.9 kDa protein sequence for mouse. Uncharacterized band(s) may appear in some lysates.
物理的形状
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABF128:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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