ABE477

Anti-BRD2 Antibody

from rabbit, purified by affinity chromatography

• 別名: Bromodomain-containing protein 2, O27.1.1, Really interesting new gene 3 protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, rat
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_001106653.1
• UniProtアクセッション番号: P25440
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BRD2(6046)

詳細

詳細

Bromodomain-containing protein 2 (BRD2) is a member of the BET subfamily of bromodomain proteins. These proteins contain two tandem N-terminal bromodomains and a single C-terminal extra-terminal domain. Studies have shown BRD2 to be associated with several regulators of transcription (eg. TFIID and Swi/Snf complexes) in addition to its involvement in guiding cell cycle gene expression through its binding to multiple E2F. BRD2 is hypothesized to function in mammalian development by regulating chromatin structure and transcription. Consistent with this hypothesis, studies have shown BRD2 to bind histone H4 via acetylated Lys12. BRD2 mutants have been associated with increased susceptibility to juvenile myoclonic epilepsy.

特異性

This antibody is expected to cross react with isoforms 1 and 2 of BRD2, based upon sequence homology.

免疫原

GST-tagged recombinant protein corresponding to a region near the C-terminus of human BRD2.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

Anti-BRD2 Antibody is a highly specific rabbit polyclonal antibody, that targets BRD2 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected BRD2 in human testis tissues.

Immunohistochemistry Analysis: A 1:2,000 and a 1:500 dilution from a representative lot detected BRD2 in rat testis tissues.

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected BRD2 in 10 µg of Jurkat cell lysate.

ターゲットの説明

~110 kDa observed. Uniprot describes two isoforms at 88 kDa (isoform 1) and 92 kDa (isoform 2). Uncharacterized band(s) may be observed in some cell lysates.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable