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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
交差性
human
交差性（ホモロジーによる予測）
bovine (based on 100% sequence homology)
テクニック
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... RECQL5(9400)
詳細
ATP-dependent DNA helicase Q5 (EC 3.6.4.12; UniProt O94762; also known as DNA helicase RecQ-like type 5, RecQ5, RecQ protein-like 5) is encoded by the RECQL5 (also known as RECQ5) gene (Gene ID 9400) in human. Human RECQ proteins (RECQ1, BLM, WRN, RECQ4, and RECQ5) constitute a family of highly conserved DNA helicases with nonredundant functions to ensure proper development. RECQ5 plays an important role in preventing both transcription-associated double-stranded breaks (DSBs) and DSBs caused by TOP1 poisons, such as camptothecin (CPT), and Recq5 deficiency leads to genome instability and cancer development in mice. RECQ5 KIX domain mediated interaction with RNA polymerase II (RNAPII) prevents RNAPII from binding transcription elongation factor TFIIS, thereby suppressing RNAPII-dependent transcription initiation and DSBs. On the other hand, RECQ5 is also known to interact with the active RNAPII (RNAPIIo) elongation complex to maintain the transcription elongation rate and prevent genome instability. In addition, RECQ5 helicase domain mediated interaction with TOP1 is reported to facilitate TOP1 K391/K436 SUMOylation, promoting TOP1-RNAPIIo association and efficient recruitment of mRNA processing factors to the transcriptionally active chromatin to prevent genome instability caused by R-loops formation.
特異性
This antiserum recognizes RECQ5 isoform Beta by targeting a sequence from the RAD51-interacting region within the helicase domain. This antiserum is expected to react with also spliced isoform 4, but not the helicase domain-truncated Alpha (RecQ5b) and Gamma (RecQ5a) spliced variants reported by UniProt (O94762).
免疫原
Epitope: RAD51 interaction domain.
Linear peptide corresponding a sequence from the RAD51 interaction domain of human RecQ5.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
細胞周期、DNA複製及び修復
細胞周期、DNA複製及び修復
This Anti-RecQ5 Antibody is validated for use in Western Blotting for the detection of RecQ5.
Western Blotting Analysis: A representative lot detected FLAG-tagged wild-type RecQ5 and a D157A (DA) ATPase-defective RecQ5 mutant construct, but not a helicase domain-truncated (a.a. 450–991 deleted) RECQ5 construct expressed in HEK293 T cells devoid of endogenous RecQ5 (Li, M., et al. (2015). Nat. Commun. 6:6720).
Western Blotting Analysis: Representative lots detected siRNA-mediated RecQ5 downregulation using whole cell lysate or chromatin fraction from human HEK293T cells. Combined treatment with siRNA and the transcription inhibitor 5,6-dichloro-beta-d-ribofuranosyl benzimidazole (DRB) further decreased cellular RecQ5 protein level (Li, M., et al. (2015). Nat. Commun. 6:6720; Li, M., et al. (2011). Mol. Cell. Biol. 31(10) 2090-2099).
Western Blotting Analysis: Representative lots detected siRNA-mediated RecQ5 downregulation using whole cell lysate or chromatin fraction from human HEK293T cells. Combined treatment with siRNA and the transcription inhibitor 5,6-dichloro-beta-d-ribofuranosyl benzimidazole (DRB) further decreased cellular RecQ5 protein level (Li, M., et al. (2015). Nat. Commun. 6:6720; Li, M., et al. (2011). Mol. Cell. Biol. 31(10) 2090-2099).
品質
Evaluated by Western Blotting in Raji nuclear extract.
Western Blotting Analysis: An 1:500 dilution of this antibody detected RecQ5 in 10 µg of Raji nuclear extract.
Western Blotting Analysis: An 1:500 dilution of this antibody detected RecQ5 in 10 µg of Raji nuclear extract.
ターゲットの説明
~120 kDa observed. 108.9 kDa (isoform Beta) and 106.1 kDa (isoform 4) calculated.
物理的形状
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE1963:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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