詳細
C-terminal-binding protein 2 (UniProt P56545; also known as CtBP2) is a nuclear protein encoded by the CTBP2 gene (Gene ID 1488) in human. CtBP1 and CtBP2 are closely related proteins that are encoded by the vertebrate genome and perform both unique and duplicative functions. Upon activation by nicotinamide adenine dinucleotide binding to their dehydrogenase domains, CtBP1 and CtBP2 exert transcriptional repression activity primarily via recruitment of a corepressor complex that consists of histone deacetylases (HDACs) and histone methyltransferases, although CtBPs can also repress transcription through HDAC-independent mechanisms. A critical function of CtBPs lies in the transcriptional repression of pro-apoptotic genes such as Bax, Puma, Bik, and Noxa. CtBP2 is essential for proper development of the nervous system. Acetylation by histone acetyl transferase p300 regulates CtBP2 activity and subcellular localization, while microenvironmental oxygen levels influences the dimerization, acetylation, and thereby the activity of CtBP2 in proliferating neural stem cells (NSCs).
特異性
Expected to react with spliced variant Ribeye (isoform 2).
免疫原
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human CtBP-2.
アプリケーション
Research Category
アポトーシス及び癌
Research Sub Category
アポトーシス-追加
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CtBP-2 in human breast cancer cells, human kidney, human ovarian cancer cells, and human prostate cancer cells.
This Anti-CtBP-2 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of CtBP-2 .
品質
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected CtBP-2 in 10 µg of HeLa cell lysate.
ターゲットの説明
~50 kDa observed. 48.9 kDa (Isoform 1; calculated); 109.2 kDa (Isoform 2/Ribeye; calculated)." ==> ~50 kDa observed. 48.9 kDa (Isoform 1; calculated); 109.2 kDa (Isoform 2/Ribeye; calculated).
物理的形状
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
