AB19167
Anti-MMP-2 Antibody, whole molecule
Chemicon®, from rabbit
別名:
72 kDa gelatinase, 72kD type IV collagenase, Gelatinase A, Matrix metalloproteinase-2, collagenase type IV-A, matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), matrix metalloproteinase 2, matrix metalloproteinase 2 (g
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
rabbit
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
bovine, rat
化学種の反応性（ホモロジーによる予測）
human, mouse
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
遺伝子情報
human ... MMP2(4313)
詳細
MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer of the stromal cells. There is a co-operation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.
特異性
AB19167 recognizes protein at 72 kDa, identified as matrix metalloproteinase 2. MMP-2 is also known as 72 kDa collagenase IV or gelatinase A. It is synthesized as a 631 amino acid proenzyme that is activated by cleavage of the first 80 amino acids. AB19167 shows no cross-reaction with (pro) and active forms of other MMPs. Cytoplasmic localization.
Reactivity with other species has not been confirmed.
免疫原
A synthetic peptide from the second half of human MMP-2.
Epitope: Whole molecule
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
MMP及び組織メタロプロテアーゼ
MMP及び組織メタロプロテアーゼ
Detect MMP-2 using this Anti-MMP-2 Antibody, whole molecule validated for use in IH(P) & WB.
Immunohistochemistry (formalin/paraffin):
10-20 µg/mL of a previous lot was kept for 30 min at RT. No special pretreatment is required for staining formalin-fixed paraffin-embedded sections.
Optimal working dilutions must be determined by the end user.
10-20 µg/mL of a previous lot was kept for 30 min at RT. No special pretreatment is required for staining formalin-fixed paraffin-embedded sections.
Optimal working dilutions must be determined by the end user.
品質
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected MMP-2 on 10 µg of PC12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected MMP-2 on 10 µg of PC12 lysates.
ターゲットの説明
72 kDa
関連事項
Replaces: 04-1048
物理的形状
Protein A chromatography
Format: Purified
Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
保管および安定性
Stable for 1 year at 2-8ºC from date of receipt.
アナリシスノート
Control
Positive control: Conditioned, serum-free medium from TPA-treated human fetal lung (HFL-1) cells. Placenta or bladder, breast, or ovarian carcinomas.
Positive control: Conditioned, serum-free medium from TPA-treated human fetal lung (HFL-1) cells. Placenta or bladder, breast, or ovarian carcinomas.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10-13 - German Storage Class 10 to 13
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Frontiers in neuroscience, 10, 593-593 (2017-01-20)
This study was to explore the mechanisms underlying 1,2-dichloroethane (1,2-DCE) induced brain edema by focusing on alteration of matrix metalloproteinase-2 (MMP-2) in rat astrocytes induced by 2-chloroethanol (2-CE), an intermediate metabolite of 1,2-DCE in vivo. Protein and mRNA levels of
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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