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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
交差性
rat, human, mouse
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
適合性
not suitable for activity/function inhibition
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... BDNF(627)
詳細
Brain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.
特異性
Brain Derived Neurotrophic Factor (BDNF). By dot blot the antibody shows less than 0.1% cross reactivity with recombinant human NT3, NT4/5 or mouse NGF.
免疫原
A 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経化学・ニューロトロフィン
神経炎症及び疼痛
神経化学・ニューロトロフィン
神経炎症及び疼痛
Immunohistochemistry:
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).
ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.
Bioassay:
Does not inhibit BDNF biological activity.
Optimal working dilutions must be determined by end user.
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).
ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.
Bioassay:
Does not inhibit BDNF biological activity.
Optimal working dilutions must be determined by end user.
This Anti-Brain Derived Neurotrophic Factor Antibody is validated for use in ELISA, IH, WB for the detection of Brain Derived Neurotrophic Factor.
品質
Evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
ターゲットの説明
18 kDa, with a homodimer at ~27-30 kDa
物理的形状
Unpurified
Rabbit polyclonal antiserum in buffer containing no preservatives. Lyophilized. Reconstitute with 50 μL of sterile distilled water.
保管および安定性
Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
Brain tissue
Brain tissue
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
does not flash
引火点(℃)
does not flash
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB1534:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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