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抗体製品の状態
purified antibody
品質水準
抗体製品タイプ
primary antibodies
メーカー／製品名
Novagen®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
詳細
Recombinase cre (UniProt: P06956; also known as Cre) is encoded by the cre gene (Gene ID: 2777477) in Escherichia phage P1 (Bacteriophage P1). Cre (Causes recombination with Cyclization recombinase), a 38 kDa DNA recombinase of the ′phage′ integrase family, is derived from the P1 bacteriophage. It catalyzes site-specific recombination and is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each bind to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals in vivo.
Specific, sensitive detection of Cre recombinase in cells and cell lysates
Supplied as purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide and 50% glycerol.
Evaluated by Western Blotting with Recombinant Cre-His.
Western Blotting Analysis: Representative lot data.Recombinant Cre-His loaded at 20 ng/lane (Lane 1) and 10 ng/lane (Lane 2) was probed with Cat. No. 69050-3, Anti-Cre (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cre (~39 kDa).
Evaluated by Western Blotting with Recombinant Cre-His.
Western Blotting Analysis: Representative lot data.Recombinant Cre-His loaded at 20 ng/lane (Lane 1) and 10 ng/lane (Lane 2) was probed with Cat. No. 69050-3, Anti-Cre (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cre (~39 kDa).
警告
Toxicity: Standard Handling (A)
法的情報
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
69050-50UL:
69050-ML:
69050-3:
69050-TML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Frontiers in genetics, 3, 255-255 (2013-01-08)
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