Purified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).
Recognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.
This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.
免疫原
Bovine Brain
bovine GFAP
アプリケーション
ELISA (0.1-0.5 µg/ml)
Immunoblotting (0.1-0.5 µg/ml)
Frozen Sections (10-50 µg/ml)
Immunoprecipitation (2-5 µg/ml)
Paraffin Sections (10-50 µg/ml, see comments)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
物理的形状
In PBS.
再構成
Following initial thaw, aliquot and freeze (-20°C).
アナリシスノート
Positive Control Human or rat brain tissues
その他情報
Product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin′s-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
Tohyama, T., et al. 1993. Am. J. Pathol.142, 871. Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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