234374

Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli

Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli, is a pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells.

• 別名: rmGM-CSF
• UNSPSCコード: 12352202
• NACRES: NA.42

特性

• リコンビナント: expressed in E. coli
• 品質水準: 100
• 詳細: Merck USA index - 14, 4538
• アッセイ: >97% (SDS-PAGE)
• フォーム: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: ≤1.0 EU/μg Endotoxin (EU/μg GM-CSF)
• 輸送温度: ambient
• 保管温度: −70°C

詳細

詳細

Recombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that stimulates the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells. Target cells include macrophages, granulocytes, and eosinophils. GM-CSF is produced by many cell types, including endothelial cells, fibroblasts, and T-lymphocytes. It can be extracted from most tissues in concentrations exceeding that of serum levels. While many cells produce GM-CSF constitutively, levels of this lymphokine increase rapidly in response to endotoxin, phorbol esters, and certain antigens.

生物化学的／生理学的作用

ED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.

再構成

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

その他情報

Anaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
Watanabe, S., et al. 1996. J. Biol. Chem.271, 12681.
Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
Gough, N.M., et al. 1985. EMBO J. 4, 645.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable