The Guard column kit contains 3 SeQuant
® ZIC
®-pHILIC guard columns including the fitting to connect to the analytical column.SeQuant
® ZIC
®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion, functional group as the silica based ZIC
®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.SeQuant
® ZIC
®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds. Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant
® ZIC
®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD). SeQuant
® ZIC
®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant
® ZIC
®-HILIC columns with identical functional groups, and SeQuant
® ZIC
®-cHILIC columns with complementary selectivity.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure:
HPLC and UHPLC Column Selection Guide Brochure:
A Practical Guide to HPLC - Method Development Guide