14-925-M

Wee1 Protein, active, 10 µg

Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.

• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 100; 300
• リコンビナント: expressed in baculovirus infected Sf21 cells
• 分子量: M_w 77 kDa
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable (kinase)
• NCBIアクセッション番号: NM_003390
• UniProtアクセッション番号: P30291
• 遺伝子情報: human ... WEE1(7465)

詳細

詳細

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ′Tyr-15′. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ′Tyr-15′ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Product Source: N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.

生物化学的／生理学的作用

Protein Target: WEE1

包装

Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.

品質

Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.

その他情報

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable