A potent, covalent, cell-permeable, irreversible inhibitor of chymotrypsin-like (50-100 nM), trypsin-like (1-5 µM), and PGPH (0.5-1 µM) activities of the 20S proteasome.
別名:
AdaAhx3L3VS, Adamantane-acetyl-(6-aminohexanoyl)₃-(leucinyl)₃-vinyl-(methyl)-sulfone, Proteasome Inhibitor XVI
A cell-permeable, peptide vinyl sulfone that acts as a potent, irreversible inhibitor of proteasome activity. Covalently modifies the catalytically active β-subunits of the proteasome. Shown to inhibit the chymotrypsin-like (10-50 nM), trypsin-like (1-5 µM), and the peptidyl glutamyl peptide hydrolyzing (PGPH) (0.5-1 µM) activities of purified 20S proteasomes from rabbit muscle. Inhibits proteasomal degradation of deglycosylated MHC HLA-A2 heavy chain in US11+ cells (~50 µM).
A potent, covalent, cell-permeable, irreversible inhibitor of chymotrypsin-like (50-100 nM), trypsin-like (1-5 µM), and PGPH (0.5-1 µM) activities of the 20S proteasome.
生物化学的／生理学的作用
Cell permeable: yes
Primary Target chymotrypsin-like activity of the 20S proteasome
Product does not compete with ATP.
Reversible: no
Target IC50: 50-100 nM against chymotrypsin-like activity of the 20S proteasome
包装
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
再構成
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
その他情報
Kessler, B.M., et al. 2001. Chem. Biol.8, 913.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
