07-2101-M

Anti-phospho PIX Antibody (Ser340)

from rabbit, purified by affinity chromatography

• 別名: Rho guanine nucleotide exchange factor (GEF) 7, PAK-interacting exchange factor beta, guanine nucleotide exchange factor 7, rho guanine nucleotide exchange factor 7, SH3 domain-containing proline-rich protein, p85, PAK3, Beta-Pix, P85COOL1, COOL-1, P85,
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology), human (based on 100% sequence homology)
• テクニック: western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_001106989
• UniProtアクセッション番号: Q9ES28
• ターゲットの翻訳後修飾: phosphorylation (pSer340)
• 遺伝子情報: human ... PAK3(5063)

詳細

詳細

p-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.

特異性

This antibody recognizes PIX phosphorylated at Ser340.

免疫原

Epitope: Phosphorylated Ser340

KLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.

アプリケーション

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

品質

Evaluated by Western Blot in PIX transfected cell lysates.

Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.

ターゲットの説明

~ 75 kDa

物理的形状

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.

アナリシスノート

Control
PIX transfected cell lysates

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1