A9853

抗アンドロゲン受容体 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗AR抗体
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01321686
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, IHC (p), IP, WB
• Citations: 19

特性

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 110 kDa (additional band may be observed at 45 kDa)
• species reactivity: rat, human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections, immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg), microarray: suitable, western blot: 1-2 μg/mL using whole extract of rat brain
• UniProt accession no.: P10275
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AR(367) ; rat ... Ar(24208)

詳細

General description

Androgens are the male sex hormones that exert biological and transcriptional effects on binding to nuclear androgen receptor (AR). Androgens in humans include androstenedione, testosterone, dihydrotestosterone and dehydroepiandrosterone. Androgen receptor is a ligand inducible nuclear receptor, expressed on brain, bone, skeletal muscles, genital organs, hair follicles and cardiovascular tissue. On binding androgen, the AR is internalized and modulates the transcriptional of target genes. Additionally, AR also exerts extra-nuclear actions wherein it rapidly induces signalling cascades of MAPK and protein kinase C. The regulation by AR results in irreversible sexual differentiation of sex organs during embryogenesis and development of sex-specific characteristics during puberty
Anti-Androgen Receptor specifically recognizes the human androgen receptor protein (110 kDa). An additional band may be observed at 45 kDa. The antibody cross-reacts with rat androgen receptor.

Immunogen

ヒトアンドロゲンレセプタ-のアミノ酸1-21に相当する進化的に保存されたペプチド。この配列は、エストロゲン, プロゲステロン, グルココルチコイドの各レセプタ-には認められません。

Application

Anti-androgen receptor antibody may be used for immunoblotting using whole rat brain extract at a recommended concentration of 1-2 μg/ml. For immunoprecipitaion from 250 μg of human MCF7 cell lysate, a concentration of 5-10 μg may be used. Immunohistochemistry may be performed on paraffin-embedded human prostate tissue sections at a working antibody concentration of 20-40 μg/ml.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-androgen receptor (C19) was used in a EMSA (electrophoretic mobility shift assay) which produced a supershift in the molecular weight of a particular DNA sequence indicating binding of the androgen receptor. [1]

Biochem/physiol Actions

抗体は、ラット由来アンドロゲンレセプタ-と交差反応性があります。

Physical form

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)