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由来生物
rabbit
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse
テクニック
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... JMJD4(65094)
詳細
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that remove specific methyl marks from histones. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
JMJD4 is a transcription factor that is a putative histone demethylase. It is highly expressed in lymphoid (spleen) and hemopoietic (bone marrow) tissues
JMJD4 is a transcription factor that is a putative histone demethylase. It is highly expressed in lymphoid (spleen) and hemopoietic (bone marrow) tissues
特異性
This antibody recognizes the N-terminal region of human JMJD4.
免疫原
Epitope: N-terminus
The immunogen was a KLH-conjugated synthetic peptide corresponding to the N-terminal region of human JMJD4.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン修飾タンパク質
エピジェネティクス
ヒストン修飾タンパク質
エピジェネティクス
ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
HeLa and NIH/3T3 cells incubated with anti-JMJD4 (N-terminus) stained positively on nuclei.
This antibody was reported by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
HeLa and NIH/3T3 cells incubated with anti-JMJD4 (N-terminus) stained positively on nuclei.
Use Anti-JMJD4 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, ELISA to detect JMJD4 (N-terminus) also known as Jumonji domain-containing protein 4.
品質
Western Blot Analysis:
1:250 dilution of this antibody detected JMJD4 on 10 µg of A431 lysate
1:250 dilution of this antibody detected JMJD4 on 10 µg of A431 lysate
ターゲットの説明
~53 kDa
物理的形状
Protein G Chromatography
Format: Purified
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
保管および安定性
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
A431 lysate
A431 lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
07-1538:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Evidence of domain swapping within the jumonji family of transcription factors.
D Balciunas et al.
Trends in biochemical sciences, 25(6), 274-276 (2000-06-06)
Toshio Ota et al.
Nature genetics, 36(1), 40-45 (2004-01-01)
As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to
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