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Merck

803561

Sigma-Aldrich

BMOE (bis-maleimidoethane)

別名:

1,1′-ethane-1,2-diylbis(1H-pyrrole-2,5-dione), N,N′-Ethylenebismaleimide, BMOE, Ethylenebismaleimide

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About This Item

実験式(ヒル表記法):
C10H8N2O4
CAS番号:
分子量:
220.18
MDL番号:
UNSPSCコード:
12352200
PubChem Substance ID:
NACRES:
NA.22

形状

powder

分子量

220.18

反応適合性

reagent type: cross-linking reagent

保管条件

desiccated

溶解性

DMSO or DMF: soluble

輸送温度

ambient

保管温度

2-8°C

SMILES記法

O=C(C=CC1=O)N1CCN2C(C=CC2=O)=O

InChI

1S/C10H8N2O4/c13-7-1-2-8(14)11(7)5-6-12-9(15)3-4-10(12)16/h1-4H,5-6H2

InChI Key

PUKLCKVOVCZYKF-UHFFFAOYSA-N

詳細

BMOE, BMB and BMH are homobifunctional, maleimide crosslinkers for conjugation between sulfhydryl groups (-SH). Such bismaleimide crosslinkers are commonly used to explore and characterize protein structure (i.e., oligomerization) or protein interactions. Because BMOE, BMB and BMH have the same reactivity but differ in length, the relative success of these three reagents in forming crosslinks between sites in a protein oligomer or interaction can assist in determining intra- and intermolecular distances.

特徴および利点

  • Reactive groups: maleimide (both ends)
  • Reactive towards: sulfhydryl groups
  • Short (8.0Å), sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 7-atom spacer arm
  • Shortest bismaleimide crosslinker available for close proximity crosslinking
  • Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
  • Noncleavable

注意

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

803561-VAR:
803561-50MG:


試験成績書(COA)

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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Z Xie et al.
The Journal of biological chemistry, 275(34), 25959-25964 (2000-06-15)
The Vibrio parahaemolyticus sodium/glucose transporter (vSGLT) is a bacterial member of the SGLT gene family. Wild-type and mutant vSGLT proteins were expressed in Escherichia coli, and transport activity was measured in intact cells and plasma membrane vesicles. Two cysteine-less vSGLT
M A Stalteri et al.
Bioconjugate chemistry, 6(2), 179-186 (1995-03-01)
Cross-linked F(ab')2 fragments derived from PR1A3, a murine monoclonal antibody used in radioimmunoscintigraphy of colorectal tumors, were produced using the bifunctional reagent bismaleimidohexane (BMH) as follows: Digestion of PR1A3 with pepsin gave F(ab')2 fragments which were purified by ion-exchange chromatography.
Kyoko Takebe et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(14), 2037-2047 (2003-11-05)
Epimorphin is a mesenchymal morphogen that has been shown to mediate epithelial-mesenchymal signaling interactions in various organs. We now show that epimorphin functions in hair follicle morphogenesis; using a novel ex vivo organ culture assay, we define a mechanism for
Yong-Kook Kwon et al.
The Journal of cell biology, 163(2), 375-384 (2003-10-22)
A thiol-reactive membrane-associated protein (TRAP) binds covalently to the cytoplasmic domain of the human insulin receptor (IR) beta-subunit when cells are treated with the homobifunctional cross-linker reagent 1,6-bismaleimidohexane. Here, TRAP was found to be phospholipase C gamma1 (PLCgamma1) by mass

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