Passa al contenuto
Merck

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18 application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53822-U

Colonna per HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

H4014

25-Hydroxycholecalciferol

≥98% (HPLC)

17937

25-Hydroxyvitamin D2

≥95.0% (HPLC)

CONDITIONS

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)

mobile phase

[A] 10mM ammonium formate in water; [B] 10mM ammonium formate in acetonitrile; (32:68, A:B)

flow rate

0.4 mL/min

pressure

1711 psi (118 bar)

column temp.

35 °C

detector

ESI(+)

injection

2 μL

sample

10 μg/mL in 25:75, water:methanol

Descrizione

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS