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Merck

Rat Embryonic Stem Cell Transgenesis.

Methods in molecular biology (Clifton, N.J.) (2023-03-31)
Elizabeth C Bryda, Hongsheng Men, Barbara J Stone
ABSTRACT

The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.

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Sigma-Aldrich
Accutase®, sterile-filtered, suitable for cell culture
Sigma-Aldrich
apo-transferrina, powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
Sodium selenite, BioReagent, suitable for cell culture, ≥98%
Eppendorf® TransferTip (ES), sterile, set of 25 ea