XNAB2RE

Extract-N-Amp^™ Blood PCR Kit

sufficient for 1000 extractions, sufficient for 5000 amplifications

• Codice UNSPSC: 12352200

Proprietà

• impiego: sufficient for 1000 extractions; sufficient for 5000 amplifications
• Colore: colorless
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, that uses an antibody based hot start and is specially formulated for amplification directly from the extract.

Genomic DNA is extracted from 10 μL of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR Mix.

The Extract-N-Amp^™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Applicazioni

Extract-N-Amp^™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples.[1][2][3][4] It has also been used in polymerase chain reaction (PCR).[5][6]

Caratteristiche e vantaggi

• Efficient 8 minute prep allows greater speed and throughput
• No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
• Simple, 3 step procedure with no special equipment required
• Hot start antibody included for highly specific PCR amplification of genomic DNA
• Compatible with multiple format (single tube or 96-well plate)
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months

• Efficient 8 minute prep allows greater speed and throughput
• No need for organic extraction, centrifugation or alcohol precipitation
• Hot start antibody for highly specific PCR amplification of genomic DNA
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months

Principio

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Altre note

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Note legali

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC