The UPS FASTA file is accessible from the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-mass-spectrometry/ups1-and-ups2-proteomic
UPS2
Proteomics Dynamic Range Standard Set
Protein Mass Spectrometry Calibration Standard
Sinonimo/i:
Dynamic Range Standards, Proteomics Standard Set
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Informazioni su questo articolo
UNSPSC Code:
12161503
EC Number:
232-650-8
NACRES:
NA.24
Form:
ready-to-use solution
Biological source:
human
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Permettici di aiutartibiological source
human
form
ready-to-use solution
quality
Protein Mass Spectrometry Calibration Standard
concentration
10.6 μg/ampule protein
technique(s)
mass spectrometry (MS): suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
Categorie correlate
1 of 4
Questo articolo | UPS1 | EMS0004 | EMS0005 |
|---|---|---|---|
| technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable | technique(s) - | technique(s) - |
| biological source human | biological source - | biological source - | biological source - |
| concentration 10.6 μg/ampule protein | concentration - | concentration - | concentration - |
| form ready-to-use solution | form ready-to-use solution | form ready-to-use solution | form ready-to-use solution |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.
Application
Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes.[2] It has also been used as a standard to spike HeLa cells for label-free quantification.[3]
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg
Related product
N° Catalogo
Descrizione
Determinazione del prezzo
signalword
Danger
target_organs
Respiratory system
Classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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Articoli
High-throughput proteomics advances with improved analysis methods and mass spectrometry.
Contenuto correlato
Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.
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Regarding SKU# UPS2, can you please send me FASTA file of 48 proteins in UPS2 product? thanks you
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