SRP5191

HSP90 α, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinonimo/i: FLJ31884, HSP86, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2
• Numero CAS: 481150-77-0
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected Sf9 cells
• Saggio: ≥70% (SDS-PAGE)
• Stato: buffered aqueous glycerol solution
• PM: ~94 kDa
• tecniche: cell culture | mammalian: suitable
• Solubilità: water: soluble
• Compatibilità: suitable for molecular biology
• N° accesso NCBI: NM_005348
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... HSP90AA1(3320)

Descrizione

Descrizione generale

Research area: Cell cycle. The gene encoding this protein is localized on human chromosome 14q32.31.[1][2]

Applicazioni

HSP90 α, His tagged human has been used to measure antibodypolyspecificity by flow cytometry.[3] It has also been used as a molecularmarker to study the effect of Temozolomide on glioblastoma cells releasedextracellular vesicles.[4]

Azioni biochim/fisiol

Heat shock protein 90α (HSP90α) is a molecular chaperone involved in the folding, assembly-disassembly, and activation of multiple types of target proteins such as kinases, steroid hormone receptors and transcription factors. HSP90 inhibitors bind to HSP90, and induce the proteasomal degradation of HSP90 client proteins. HSP90α is an important mediator of cancer cell invasion and is expressed extracellularly on fibrosarcoma and breast cancer cells where it interacts with MMP2 (matrix metalloproteinase-2).[5][6]HSP90α are important molecular chaperones involved in signal transduction, cell cycle control, stress management, folding, degradation, and transport of proteins.[1]

Stato fisico

Supplied in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Nota sulla preparazione

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H360D
• Consigli di prudenza: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable