SRP0229

WWP1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥15% (SDS-PAGE)

• Sinonimo/i: AIP5, Atrophin-1 interacting protein 5, NEDD4-like E3 ubiquitin-protein ligase, TGIF-interacting ubiquitin ligase 1 (Tiul1), WW domain containing E3 ubiquitin protein ligase 1
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥15% (SDS-PAGE)
• Stato: aqueous solution
• PM: 106 kDa
• Confezionamento: pkg of 20 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_007013
• N° accesso UniProt: Q9H0M0
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... WWP1(11059)

Descrizione

Descrizione generale

Human WWP1 (GenBank Accession No. NM_007013), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definizione di unità

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.