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SRP0151

Sigma-Aldrich

SMYD2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Sinonimo/i:

KMT3C, Lysine N-methyltransferase 3C, SET and MYND domain containing 2, ZMYND14, Zinc finger, MYND domain containing 14

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in baculovirus infected insect cells

Saggio

≥80% (SDS-PAGE)

Forma fisica

aqueous solution

PM

49.7 kDa

Confezionamento

pkg of 20 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

Concentrazione

>0.02 mg/mL

N° accesso NCBI

NM_020197

N° accesso UniProt

Q9NRG4

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... SMYD2(56950)

Descrizione generale

SET and MYND domain containing 2 (SMYD2) is encoded by the gene mapped to human chromosome 1q32.3-q41. The encoded protein belongs to SMYD family of protein lysine methyltransferases.
Human SMYD2 (GenBank Accession No. NM_020197), full length with N-terminal DDDDK tag (FLAG), MW = 49.7kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Azioni biochim/fisiol

SET and MYND domain containing 2 (SMYD2) might act as a putative oncogene by methylating p53 and reducing its tumor suppressive role. Overexpression of SMYD2 gene is associated with pathogenesis and metastasis of oesophageal cancer, squamous cell carcinoma (ESCC). Therefore, it is considered as a potent therapeutic target for ESCC. SMYD2 has capacity to catalyze the methylation of histone and non-histone substrates. AZ505 acts as a potent and selective inhibitor of SMYD2 and aids in the improvement of novel cancer treatment.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Certificati d'analisi (COA)

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Repression of p53 activity by Smyd2-mediated methylation.
Huang J
Nature, 444(7119), 629-632 (2006)
Makoto Nakakido et al.
Neoplasia (New York, N.Y.), 17(4), 367-373 (2015-05-01)
Phosphatase and tensin homologue (PTEN), one of the well-characterized tumor suppressor proteins, counteracts the phosphatidylinositol 3-kinase-AKT pathway through its unique lipid phosphatase activity. The functions of PTEN are regulated by a variety of posttranslational modifications such as acetylation, oxidation, ubiquitylation
Prediction of CCND1 amplification using plasma DNA as a prognostic marker in oesophageal squamous cell carcinoma.
Takeshita H
British Journal of Cancer, 102(9), 1378-1383 (2010)
Overexpression of SMYD2 relates to tumor cell proliferation and malignant outcome of esophageal squamous cell carcinoma.
Komatsu S
Carcinogenesis, 30(7), 1139-1146 (2009)
Structural basis of substrate methylation and inhibition of SMYD2.
Ferguson AD
Structure, 19(9), 1262-1273 (2011)

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