Passa al contenuto
Merck
Tutte le immagini(1)

Documenti

SHC312V

Sigma-Aldrich

MISSION® 1X LacO Inducible Non-Target shRNA Control Transduction Particles

Does not target any known genes

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
41106609
NACRES:
NA.51

Livello qualitativo

100

Nome Commerciale

MISSION®

Concentrazione

≥1x106 VP/ml (via p24 assay)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Categorie correlate

Descrizione generale

Sigma® is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC.
The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-Β-D-thio-galactoside), an analogue of lactose, LacI binds to LacO preventing expression of the shRNA. When IPTG is present, the allosteric LacI repressor changes conformation, releasing itself from lacO modified human U6 promoter, and subsequently allows expression of the shRNA.
The MISSION 1x LacO Inducible Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. 200uL of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.

Applicazioni

To see more application data, protocols, vector maps visit sigma.com/shrna.

Note legali

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Sigma is a registered trademark of Sigma-Aldrich Co. LLC

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Articoli

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Contenuto correlato

Inducible shRNA Vectors

Explore the benefits of IPTG-inducible vectors through performance data. These vectors offer regulated expression, which is important when studying essential and lethal genes.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.