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S6402

Sigma-Aldrich

Streptavidin−Cy3 from Streptomyces avidinii

buffered aqueous solution

Sinonimo/i:

Streptavidin Cy3

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Coniugato

CY3 conjugate

Forma fisica

buffered aqueous solution

Grado di funzionalizzazione

3-9 mol fluorochrome per mol protein

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.

Applicazioni

Streptavidin−Cy3 from Streptomyces avidinii has been used:
  • in double immunofluorescence staining to detect biotinylated lectin
  • for insulin and C-peptide staining in immunofluorescence analysis
  • in double immunofluorescence to verify the presence of cells filled with biocytin
  • in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
  • with the traditional rhodamine filter arrangements due to the spectral overlap

Caratteristiche e vantaggi

Spectral Characteristics of Cy3 Dye:
  • Absorbance Max 552 nm
  • Emission Max 565 nm
  • Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Note legali

Cy3 is a trademark of Cytiva

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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