P3026
Pectolyase from Aspergillus japonicus
lyophilized powder, ≥0.3 units/mg solid
Sinonimo/i:
Pectolytic enzyme
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About This Item
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
Aspergillus sp. (A. japonicus)
Forma fisica
lyophilized powder
Attività specifica
≥0.3 units/mg solid
PM
50 kDa
Caratteristiche più verdi
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Categoria alternativa più verde
, Enabling
Temperatura di conservazione
2-8°C
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Descrizione generale
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.
Applicazioni
Pectolyase from Aspergillus japonicus has been used:
- in the lysis of chlorella cells for protoplast generation
- as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation
- to induce reorganization of Arabidopsis root cap cell architecture
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Azioni biochim/fisiol
Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis.
Altre note
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Definizione di unità
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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I clienti hanno visto anche
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P J De Lange et al.
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Pectic enzymes in pectolyase: separation, characterization, and induction of ethylene in fruits
Baldwin EA and Pressey R
Plant Physiology, 90(1), 191-196 (1989)
Protocol for the preparation of Arabidopsis meiotic chromosome spreads and fluorescent in situ hybridization
Bolanos-Villegas P, et al.
The Plant Journal, 4, e1102-e1102 (2014)
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