OGS91
PSF-CMV-NH2-V5-EKT-NCOI - N-TERMINAL V5 TAG PLASMID
plasmid vector for molecular cloning
Sinonimo/i:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.85
Tag
V5 tagged
Stato
buffered aqueous solution
PM
size 4280 bp
Selezione batterica
kanamycin
Origine di replicazione
pUC (500 copies)
Clivaggio proteico
no cleavage
Posizione del tag peptidico
N-terminal
Promotore
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
Gene reporter
none
Condizioni di spedizione
ambient
Temperatura di conservazione
−20°C
Descrizione generale
Plasmid vector adds a V5 epitope tag to the N-terminus of a protein that is encoded within the multiple cloning site. This tag allows the detection and purification of a tagged protein using antibodies raised against the Influenza V5 epitope. The V5 tag coding sequence is GKPIPNPLLGLDST. There is an enterokinase cleavage site (DDDDK) immediately downstream of the V5 tag that can be used to remove the V5 tag from a purified protein. It cleaves after the lysine residue.
Promoter Expression Level: PSF-CMV-NH2-V5-EKT-NCOI - N-terminal V5 tag plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Promoter Expression Level: PSF-CMV-NH2-V5-EKT-NCOI - N-terminal V5 tag plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Applicazioni
Molecular cloning vector has been designed to allow the addition of a peptide tag to the end of a protein of interest using standard cloning techniques.Multiple Cloning Site Notes:
There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI however the tag resides between the NotI and HindIII sites. There are Shine-Dalgarno sequences and KOZAK sequences aligned with the start codon of the peptide tag.
The ClaI to NheI sites have other functions such as adding C-terminal peptide tags second promoters or IRES expression components. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of C-terminal peptide tags sequences if the stop codon is placed in this position.
There is a start codon in the NcoI site can be removed by digestion with KpnI if required. The MCS for gene insertions extends from NotI to XbaI however the tag resides between the NotI and HindIII sites. There are Shine-Dalgarno sequences and KOZAK sequences aligned with the start codon of the peptide tag.
The ClaI to NheI sites have other functions such as adding C-terminal peptide tags second promoters or IRES expression components. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of C-terminal peptide tags sequences if the stop codon is placed in this position.
Sequenza
To view sequence information for this product, please visit the product page
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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