O5003
OPI Media Supplement
Hybri-Max™, γ-irradiated, powder, suitable for hybridoma
Sinonimo/i:
OPI Media for cell culture
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.75
Prodotti consigliati
Grado
Hybri-Max™
Livello qualitativo
Sterilità
γ-irradiated
Forma fisica
powder
Concentrazione
18-40 mg/vial
tecniche
cell culture | hybridoma: suitable
Impurezze
endotoxin, tested
Temperatura di conservazione
−20°C
Applicazioni
OPI Media Supplement has been used to grow hybridoma cell lines from XenoMouse strains and BALB/c female mice.
Altre note
Formulated with: 0.15 g oxaloacetate, 0.05 g pyruvate, and 0.0082 g insulin.
Ricostituzione
Reconstitute contents of vial with 10 mL sterile water. Do not use cell culture medium for initial reconstitution. Each vial sufficient to prepare 1 liter of medium. Final working concentration: 1 mM oxaloacetate, 0.45 mM pyruvate, 0.2 U/ml insulin.
Note legali
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Experimental and data analysis protocols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface plasmon resonance biosensor platforms. The screening methods involved capturing antibodies from crude supernatants using Fc-specific antibody surfaces and monitoring antigen binding at a
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Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
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