A5159

Aminopterin

Hybri-Max^™, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Sinonimo/i: (S)-2-{4-[(2,4-Diaminopteridin-6-yl)methylamino]benzamido}pentanedioic acid, 4-Amino-PGA, 4-Aminofolic acid, 4-Aminopteroyl-L-glutamic acid
• Formula empirica (notazione di Hill): C₁₉H₂₀N₈O₅
• Numero CAS: 54-62-6
• Peso molecolare: 440.41
• Beilstein: 69045
• Numero CE: 200-209-9
• Numero MDL: MFCD00036692
• Codice UNSPSC: 12352205
• ID PubChem: 24890950
• NACRES: NA.75

Proprietà

• Origine biologica: synthetic
• Grado: Hybri-Max^™
• Sterilità: γ-irradiated
• Nome Commerciale: BioXtra
• Stato: lyophilized powder
• Concentrazione: 50 ×
• tecniche: cell culture | hybridoma: suitable
• Impurezze: endotoxin, tested
• Solubilità: 10 mL/vial, clear, red (in TC Medium)
• ε (coefficiente d’estinzione): 24,500 at 282 nm in 0.1 M NaOH at 1 M; 25,700 at 261 nm in 0.1 M NaOH at 1 M; 8,100 at 373 nm in 0.1 M NaOH at 1 M
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• Stringa SMILE: Nc1nc(N)c2nc(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)cnc2n1
• InChI: 1S/C19H20N8O5/c20-15-14-16(27-19(21)26-15)23-8-11(24-14)7-22-10-3-1-9(2-4-10)17(30)25-12(18(31)32)5-6-13(28)29/h1-4,8,12,22H,5-7H2,(H,25,30)(H,28,29)(H,31,32)(H4,20,21,23,26,27)/t12-/m0/s1; TVZGACDUOSZQKY-LBPRGKRZSA-N
• Informazioni sul gene: human ... FPGS(2356) ; mouse ... Fpgs(14287)

Descrizione

Applicazioni

Aminopterin concentrate supplement for hybridoma cell culture applications.

Azioni biochim/fisiol

Folic acid antagonist that binds to dihydrofolate reductase and inhibits its activity.

Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.

Linkage

More potent, but more toxic, than methotrexate.

Ricostituzione

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 0.4 μM aminopterin.

Note legali

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H300,H315,H319,H341,H360FD
• Consigli di prudenza: P202 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 1 Oral - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2
• Codice della classe di stoccaggio: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges